A purchase order can become a legally binding contract once the seller accepts it. Here is when a PO is binding, and how it differs from a purchase agreement.

A purchase order (PO) can be a legally binding contract, but it does not start out as one. A purchase order becomes a contract at the moment the seller accepts it, either explicitly or by fulfilling the order. Until then, it is an offer to buy.
Quick answer: a purchase order is not automatically a contract when issued. It becomes binding once the seller accepts it, at which point its terms are enforceable.
A purchase order follows the basic rules of contract formation. It is an offer from the buyer. When the seller accepts that offer, by confirming the order or beginning to fulfill it, the necessary elements of a contract are in place: offer, acceptance, and consideration. At that point the purchase order is a binding contract.
For routine orders, a purchase order is often enough. For larger or higher-risk purchases, a purchase agreement gives both sides more protection.
Because an accepted purchase order is enforceable, the terms printed on it matter. Standard terms and conditions attached to a purchase order become part of the contract. Tracking those terms across thousands of orders is part of why contract management is the demanding side of procurement, and why teams move toward managing agreements as connected data. A purchase order is one of many types of contracts a business relies on.
Is a purchase order legally binding?
Not on its own. A purchase order becomes legally binding once the seller accepts it, either by confirming or by fulfilling the order.
What is the difference between a purchase order and a contract?
A purchase order is a buyer's offer to purchase. It becomes a contract when accepted. A contract such as a purchase agreement is typically negotiated and signed by both parties from the outset.
Does a purchase order need a signature to be binding?
No. Acceptance can occur through confirmation or performance, not only a signature, though signed acknowledgment provides clearer evidence.